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Figure 1 | Retrovirology

Figure 1

From: Functional analysis of human T lymphotropic virus type 2 Tax proteins

Figure 1

Relative transactivation phenotypes and expression levels of Tax 2A proteins Mo, Lor, Gar and Tax 2B proteins. Jurkat (A) and 293T cells (B) were co-transfected with 250 ng of empty or Tax expression plasmids together with 1ug of either the HTLV-1 LTR or NFkB luciferase reporter plasmids and 50 ng of pRL-TK. Reporter activities were measured using the Dual Luciferase Assay system (Promega) and were normalised to Renilla luciferase values. The values indicate the mean of four independent experiments normalised to Tax 2B (100%) and the error bars represent the SEM. A minimum of three replicates of each Tax construct was included in the calculation of each mean activity. (C) Western blot analysis of lysates from 293T cells transfected with 250 ng of the indicated plasmids. Tax proteins were detected using an anti-HIS antibody. Tubulin was used as a loading control and was detected using anti-Tubulin.

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