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Figure 3 | Retrovirology

Figure 3

From: Factor correction as a tool to eliminate between-session variation in replicate experiments: application to molecular biology and retrovirology

Figure 3

Virus production of HIV-1 variants. The HIV-1 molecular clone LAI and derivatives with a modified mechanism of transcription regulation [13] and variation in the viral Tat gene were transfected into C33A cells. Virus production was measured at two days after transfection. The experiment was repeated seven times. A: mean values with standard deviation of observed data. B: normalisation of the data with the WT construct set at 100% in each session. C: corrected data after standardisation. D: data after removal of between-session variation with factor correction. WT: HIV-rtTA construct with wild-type Tat gene; A-D: HIV-rtTA variants with mutated Tat genes (to be described elsewhere); LAI: HIV-LAI proviral clone with unmodified mechanism of transcription regulation. An asterisk indicates a statistically significant difference between the virus variant and WT (t-test; P < 0.05). The number of observations per variant is 8.

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