Figure 5

Cell-to-cell fusion inhibition by the Rgp41A. HeLa-Env/LAI, HeLa-Env/NDK, and HeLa-Env/ADA were incubated with target cells expressing the HIV receptors in the presence of PBS, Rgp41A (0.03 μg/μl), Rgp41A solubilization buffer or T-20 (0.2 μg/ml). Syncytia formation was evaluated by measuring the β-galactosidase activity after lysis of the cells. The results are expressed as percentage of the β-galactosidase activity observed in the control with PBS. Results represent the average of three independent experiments performed in duplicate. Standard deviations are indicated by error bars.