Figure 3

In vitro interaction between Rgp41 proteins and soluble monomeric gp120. Ninety-six well plates were coated with increasing doses of recombinant gp41 proteins (0, 50, 100 or 200 ng) and then incubated with 2 ng of soluble gp120. Bound gp120 was revealed using specific anti-gp120 antibodies and HRP-conjugated secondary antibodies. Black bars: wells coated with Rgp41A, gray bars: wells coated with Rgp41B. Results represent the average of two independent experiments. Standard deviations are indicated by error bars.