Figure 1

Gp41-derived recombinant proteins. A. Schematic representation of Rgp41A (N59(L7)C54) and Rgp41B (N54(L7)C47) synthetic trimeric peptides, derived from the HIV-1 gp41 ectodomain. Grey boxes represent the position of the N36 and C34 peptides. For comparison, the sequence of T20 is also indicated. B. Characterization of recombinant proteins. SDS-PAGE analysis and gel filtration on a Superdex 75 column. The elution profiles of Rgp41 proteins were compared to a calibration curve realized with standard globular proteins. An elution volume of 10.0 ml corresponds to an apparent molecular weight of 50 kDa.