Figure 7
Transactivation of B and BF LTRs by clade B and BF specific Tat proteins (A: CRF12_BF; B: ARCH003 and ARCH005). 293T cells were transfected with GFP reporter constructs containing HIV-1 clade-specific LTRs in absence or presence of expression plasmids encoding Tat B(NL4-3) or TatBF(ARMA159). Relative transactivation is expressed as mean fluorescence obtained by FACS analysis. Cells transfected only with LTRs constructs were used as negative controls. Error bars indicate standard errors.