Coordinate enhancement of transgene transcription and translation in a lentiviral vector

Retrovirology

Table 1 The combination of PCE and CMV IE increased Luc activity in transfected 293 cells

	Luc activity (Relative Light Units)^a
	Replicate Experiment
Vector	1	2	3	4
U3-Luc	2,955 ± 171 (1)^b	3,809 ± 207 (1)	3,605 ± 3 (1)	3,796 ± 700 (1)
PCE-Luc	20,810 ± 559 (7.0)*	19,644 ± 343 (5.1)*	14,756 ± 382 (4.0)*	15,110 ± 842 (3.9)*
IE-U3-Luc	10,490 ± 159 (3.5)	13,174 ± 228 (3.4)	12,945 ± 2,677 (3.6)	16,676 ± 435 (4.4)
IE-PCE-Luc	49,870 ± 28 (16.8)*	48,085 ± 90 (12.6)*	39,485 ± 7,303 (11)*	50,424 ± 1,952 (13)*

^a Two-days post-transfection with the indicated vector, which encodes firefly Luciferase (Luc) and Renilla luciferase control plasmid, total cellular protein was harvested and relative Luciferase levels were measured by chemiluminescence assay. Luc level was standardized to cotransfected Renilla Luc and results are presented of four independent experiments performed in duplicate or triplicate. ANOVA with repeated measures determined that increases in response to PCE and IE were significant as indicated by * (p-values of 0.0008 and < 0.0001, respectively).
^b (), Fold difference relative to U3-luc vector.

ISSN: 1742-4690