Figure 5
From: Coordinate enhancement of transgene transcription and translation in a lentiviral vector
The half-life of luc RNA is not decreased by PCE. 293 cells transduced with PCE-Luc, U3-Luc, IE-PCE-Luc or IE-U3-Luc were treated with actinomycin D (ActD) for indicated time intervals and total RNA was isolated and subjected to Northern blot analysis with luc or gapdh complementary DNA probes. (A,B) Northern blot results from a representative of two replicate experiments. The abundance of PCE-containing RNAs is lower than PCE-lacking RNAs, while the abundance of gapdh loading control was similar. (C,D) Decay curves were generated with luc RNA signal standardized to gapdh. The presence of PCE did not reduce the stability of luc RNA.