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Figure 4 | Retrovirology

Figure 4

From: Coordinate enhancement of transgene transcription and translation in a lentiviral vector

Figure 4

Western blots demonstrate appropriate subcellular fractionation of transduced cells. Equivalent amounts of each nuclear or cytoplasmic fraction were subjected to immunoblot with antiserum against the nuclear protein histone H1; the cytoplasmic protein β-tubulin; and loading control β-actin, which is distributed in the nucleus and cytoplasm. The results determined that similar levels of protein were loaded and verified effective subcellular fractionation.

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