Figure 3
From: Coordinate enhancement of transgene transcription and translation in a lentiviral vector
PCE increases translational efficiency of lentiviral vector transgene RNA in transduced cells. (A) Northern blot analysis of total cellular RNA that was isolated 48 hours post-transduction and hybridized with a probe complementary to luc or gapdh loading control (B) Graphic representation of the luc RNA in transduced cells. Black bars indicate translational efficiency relative to the corresponding U3 vector. Translational efficiency is defined as the ratio of Luc activity to luc mRNA.