Figure 2
From: Coordinate enhancement of transgene transcription and translation in a lentiviral vector
PCE increases lentiviral vector transgene activity in transfected cells. (A) Northern blot analysis of total RNA that was isolated 48 hrs after transfection with the indicated vector and hybridized with DNA probe complementary to luc open reading frame or gapdh loading control. The gapdh level was used to standardized minor differences in RNA sample concentration. (B) Graphic representation of data from (A) with white bars indicating the luc RNA levels standardized to gapdh loading control. Black bars indicate translational efficiency relative to the corresponding U3 vector. Translational efficiency is defined as the ratio of Luc activity in these samples to luc mRNA in (A).