Figure 2

Efficient HIV-1 replication is dependent upon the presence of a suboptimal signal at 5'ss D2. (A) Reverse transcriptase activity of cell-free supernatants from 293T cells transfected with either NLD2UP or NEVM mutants. Asterisks indicate a significant difference when compared to mock transfected cells from three independent experiments (p < 0.01 by Student's t-test). (B) HIV-1 p24 Gag production from transfected 293T cells was measured by subjecting ten-fold serial dilutions of cell-free supernatants to Western blot analysis using serum from an HIV-1 infected patient. (C and D) Protein from transfected 293T cells was subjected to Western blot analysis using serum from an HIV-1 infected patient or antibodies to the indicated cellular or viral gene product.