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Figure 6 | Retrovirology

Figure 6

From: Kaposi's sarcoma associated herpes virus-encoded viral FLICE inhibitory protein activates transcription from HIV-1 Long Terminal Repeat via the classical NF-κB pathway and functionally cooperates with Tat

Figure 6

Effect of HIV Tat protein on K13-induced HIV-1 LTR activation. A-C. 293T cells were transfected with an empty vector (pCDNA3) or K13 along with different HIV LTR/luciferase reporter constructs and β-galactosidase as described in Fig. 1A. The values shown are averages (Mean ± S.E.) of one representative experiment out of three in which each transfection was performed in duplicate. A, Wild type HIV-1 LTR reporter; B, HIV-1 LTR reporter with deletion of Tat-binding site (nucleotides +23 to +25); C. HIV-1 LTR reporter lacking the NF-κB binding-sites. D. Dose-response analysis of Tat-induced HIV-1 LTR activation. E. K13 and Tat synergistically activate HIV-1 LTR. The experiment was performed as described for Fig. 6 A. The total amount of transfected DNA was kept constant by adding empty vector.

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