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Figure 3 | Retrovirology

Figure 3

From: Kaposi's sarcoma associated herpes virus-encoded viral FLICE inhibitory protein activates transcription from HIV-1 Long Terminal Repeat via the classical NF-κB pathway and functionally cooperates with Tat

Figure 3

Electrophoretic mobility shift assay. A. The nuclear extract from Jurkat cells stably expressing an empty vector (lane 1) and K13 (lanes 2–4) were used for EMSA. The position of the induced HIV-1 LTR complex is marked with an asterisk. The specificity of the complex is demonstrated by competition with excess cold HIV-1 LTR probe (lane 3) and a nonspecific (N.S.) probe (lane 4), respectively. B. A supershift assay showing the subunit composition of K13-induced NF-κB subunits bound to HIV-1 LTR. The supershift assay was performed using a control rabbit antisera (lane 3), control mouse antisera (lane 4), or antisera against p50 (lane 5), p65 (lane 6), p52 (lane 7), Rel B (lane 8) and c-Rel (lane 9) subunits of NF-κB, respectively. The position of the induced HIV-1 LTR complex is marked with an asterisk, while the super-shifted bands are marked by arrowheads.

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Retrovirology

ISSN: 1742-4690

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