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Figure 1 | Retrovirology

Figure 1

From: Targeted infection of HIV-1 Env expressing cells by HIV(CD4/CXCR4) vectors reveals a potential new rationale for HIV-1 mediated down-modulation of CD4

Figure 1

Chimeric CD4 proteins. (A) Membrane anchors. The CD4 ectodomain of CD4 (1) was linked to five heterologous membrane anchors: the glycosylphosphatidyl inositol region of the DAF protein (2), the transmembrane and cytoplasmic domains of either HIV-1 Env (5) or the VSV G (6), or the entire gp41 region of HIV-1 Env with (3) or without (4) the proteolytic cleavage site. (B) Western blot analysis of CD4 and the chimeric CD4 proteins. The six proteins were expressed from the CMV promoter in pCR3 after transfection of Hek293 cells and analyzed by Western blot using anti-CD4 antibodies. (C) Intracellular and cell surface expression. Intracellular expression of chimeric CD4 proteins after DNA transfection in HeLa cells was evaluated using immunofluorescent staining of fixed cells after cell permeabilization and compared to cell surface expression of CD4 without cell permeabilization.

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Retrovirology

ISSN: 1742-4690

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