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Table 1 Real-time PCR analysis of hTERT, POT1, TERF1 and TERF2 gene expression upon activation in freshly isolated CD4+ T lymphocytes.

From: A balanced transcription between telomerase and the telomeric DNA-binding proteins TRF1, TRF2 and Pot1 in resting, activated, HTLV-1-transformed and Tax-expressing human T lymphocytes

  Unstimulated 48 h-Stimulated Fold Activation
hTERT 0.019* 0.43 ± 0.02 22.6
Pot1 10.16 ± 0.02 3.21 ± 0.05 (-) 3.1
TRF1 2.08 ± 0.08 0.70 ± 0.09 (-) 2.9
TRF2 16.69 ± 8.4 4.24 ± 0.03 (-) 3.9
  1. *Values are corrected for the expression of the human housekeeping gene PBGD in cells of each population. Results are expressed as the amount of indicated mRNA relative to PBGD and compared to that found in Jurkat cells referred as 1. Standard deviations are from two determinations performed in triplicate.