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Table 1 Real-time PCR analysis of hTERT, POT1, TERF1 and TERF2 gene expression upon activation in freshly isolated CD4+ T lymphocytes.

From: A balanced transcription between telomerase and the telomeric DNA-binding proteins TRF1, TRF2 and Pot1 in resting, activated, HTLV-1-transformed and Tax-expressing human T lymphocytes

 

Unstimulated

48 h-Stimulated

Fold Activation

hTERT

0.019*

0.43 ± 0.02

22.6

Pot1

10.16 ± 0.02

3.21 ± 0.05

(-) 3.1

TRF1

2.08 ± 0.08

0.70 ± 0.09

(-) 2.9

TRF2

16.69 ± 8.4

4.24 ± 0.03

(-) 3.9

  1. *Values are corrected for the expression of the human housekeeping gene PBGD in cells of each population. Results are expressed as the amount of indicated mRNA relative to PBGD and compared to that found in Jurkat cells referred as 1. Standard deviations are from two determinations performed in triplicate.