Figure 2

Inhibition of recombinant lentiviral vector generation by Tat-P peptide. Panel A. To visualize the genetically generated pseudo HIV particles, 293T cells were co-transfected for 24 hours with 3 plasmids: pCMV Δ8.91, pMD VSV-G, and pHR'GFP. Then 200 μM of Tat-P and Con-P1 peptides, and the same amount of PBS, were added to the 293T cells for 12 hours. The cells were then fixed and sectioned for EM imaging (Magnification × 60,000). Arrows indicate the virus particles. Panel B and Panel C. To examine the Tat-P inhibition of HIV derived recombinant lentiviral vectors, 293T cells were cotransfected with 3 plasmids for 24 hours. The medium was replaced with DMEM containing 200 μM of Tat-P, Con-P1, Con-P2 peptides or PBS. Six hours later, the supernatants containing the viral particles were collected and the vector titers were determined. A representative from three individual experiments is presented. Panel D. Percent inhibition was calculated using the formula (1-titer in the presence of peptide/titer in the presence of PBS) × 100.