Figure 5
From: Presence of a functional but dispensable Nuclear Export Signal in the HTLV-2 Tax protein
Within the context of a full length Tax2 protein, the presence of a functional NES2 domain is dispensable for the protein localization. (A): HeLa cells were transiently transfected with the GFP-Tax1, GFP-Tax2 and the different GFP-Tax2 mutants plasmids. Eighteen hours post transfection, the cells were washed, fixed, mounted with DAPI-containing medium and visualized with a Zeiss Axioplan 2 imaging microscope X40 using a Zeiss Axiocam HRc (color) camera and the Zeiss Apotome software. Images of cells that are representative of the entire population are shown. (B): Western-blot analysis of GFP and GFP-NES proteins. 293T cell lysates (70 μg) were subjected to electrophoresis on a 10 % TG gel and probed with GFP or β-tubulin antibodies.