Figure 5
From: Silencing of human T-cell leukemia virus type I gene transcription by epigenetic mechanisms
DNA methylation of provirus is not associated with methylated CpG sites in the genome. Integration sites of HTLV-I provirus in leukemic cells have been determined by inverse PCR, and then DNA methylation in genome has been analyzed by sodium bisulfite sequencing. DNA methylation of 5'-LTR was also analyzed by sodium bisulfite sequencing method. Vertical bars represent CpG sites. Open circle indicates unmethylated CpG site, and closed one means methylated CpG site. N: normal PBMCs from non-carrier donor.