Figure 3
From: Silencing of human T-cell leukemia virus type I gene transcription by epigenetic mechanisms
Comparison of the DNA methylation in carriers and ATL cases. A. DNA methylation at eight different regions in the HTLV-I provirus was compared between carriers (C) and ATL cases (A). DNA methylation was quantified by COBRA in 20 carriers and 20 ATL cases. Each sample was analyzed three times by COBRA at each site, and circles indicate mean values of DNA methylation. The differences of DNA methylation are statistically significant in the gag, pol and env regions by the Mann-Whitney's U-test. Horizontal bars represent median of DNA methylation in each group. B. The relation between tax gene transcription and DNA methylation of 5'-LTR in the fresh ATL cells has been shown. DNA methylation of 5'-LTR was quantified by COBRA assay and the tax gene transcripts were detected by RT-PCR.