Figure 2
From: Silencing of human T-cell leukemia virus type I gene transcription by epigenetic mechanisms
DNA methylation in ATL cell lines, HTLV-I carriers and ATL cases. The tax gene transcription in ATL cell lines was studied by RT-PCR (A), and the expression of GAPDH gene has been used as a control. DNA methylation throughout the HTLV-I provirus was studied by COBRA in tax gene-expressing (B) and non-expressing cell lines (C). Furthermore, DNA methylation was also analyzed in 20 carriers and 20 ATL cases by COBRA, and representative patterns of DNA methylation are shown in D. The number of HTLV-I provirus has been analyzed by Southern blot method, and shown in the parenthesis (B, C and D). Each bar indicates the extent of DNA methylation that was calculated by COBRA.