Figure 6

In vivo transduction of cells in other brain regions. Immunohistochemical staining reveals pREV-HW3 transduced cells in and adjacent to the ependymal walls throughout the ventricular system including in the lateral ventricles (A, B, C and D). PLAP expressing cells are identified in the ependymal wall, and in the adjacent brain parenchyma (arrow heads in A). Transduced cells are not reactive to anti-HU antibody (B). Double labeling for PLAP (E) and GFAP (F) showed that the PLAP transgene predominantly co-localizes with GFAP in cells (arrow in E and F) and processes (arrow heads). Analysis of the overlying cortex showed cells co-expressing PLAP (H) and HU (I) in the most superficial layers of cortex (arrows in H and I). Many of the transduced cells in this region did not express HU (white arrow heads in H, I, J and K). Note the absence of HU staining in small piece of attached meninges (magenta arrow head in H, I, J and K). DNA staining with bis-benzimide is shown C, G and J and phase contrast of the section in A, B and C, and the section in H, I and J, are shown in D and K, respectively. Scale bars correspond to 75 μm (C, G) and 100 μm (J).