Figure 3
Dissemination of injected MLV vector producer cells from the injection site. Panels A, B, C and D are micrographs from brain sections of an animal injected with pREV-HW3 vector producer cells, while panels E, F, G and H are micrographs from brain sections of an animal injected with helper cells transfected with pREV-HW1. Panel B shows the region of A bordered by a black rectangle at greater magnification. PLAP histochemical staining shows cells in different sites including the 4th ventricle and its lateral recesses and in the perimedian sulcus (A, B and G, respectively) and trapped in the subarachnoid space, between the meninges on the surface of the brain parenchyma, particularly in the region of the basal artery (C, D and F). Similar distributions were obtained with vector producing cells (A, B, C and D) and helper cells transfected with pREV-HW1 (E, F, G and H). PMS, perimedian sulcus. Size bars indicate 200 μm (A and G), 100 μm (B), 125 μm (C and D), 450 μm (E), 360 μm (F) and 250 μm (H).