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Figure 2 | Retrovirology

Figure 2

From: Dicistronic MLV-retroviral vectors transduce neural precursors in vivoand co-express two genes in their differentiated neuronal progeny

Figure 2

Location of injected MLV-vector producer cells. Injected helper cells were found mainly in the hind brain beneath the caudal cerebellum. The red line in A indicates the position in the rostro-caudal axis, and the red rectangle in B shows the location of the photomicro graph presented in C. The sections in D, F and G correspond to the region bordered by the white rectangle shown in C. E shows the region bordered by the white rectangle in D at higher magnificationInjected cells were easily distinguished from the surrounding tissue on the basis of transgene expression shown by immunofluorescence for Neo (C) or PLAP (F). Brightly stained cells express transgene. Alternatively PLAP activity could be revealed by histochemistry in which case the dark cells express PLAP (D and E). It was also possible to identify injected producer cells by staining DNA as shown in field G which corresponds to the same field as D stained with bis-benzimide. The nuclei of injected cells appeared to fluoresce more brightly than brain cell nuclei and were elongated in contrast to the round nuclei typical of neural cells. Macroscopically, injected cells appeared as disorganized patches in the surrounding brain parenchyma. PMS, perimedian sulcus. Scale bars indicate 200 μm (C and D), 20 μm (E, F and G)

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