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Figure 1 | Retrovirology

Figure 1

From: Dicistronic MLV-retroviral vectors transduce neural precursors in vivoand co-express two genes in their differentiated neuronal progeny

Figure 1

Schematic representation of dicistronic MLV vectors. Vectors used in this study have been previously described [22, 23]. MLV E+ corresponds to the enhanced packaging region of MLV and the internal ribosome entry signal (IRES). pREV-HW3 and pEMCV-CBT4 contain two IRESes [22, 23]. For both vectors the first is that of MLV and drives expression of human placental alkaline phosphatase (PLAP). In the pREV-HW3 the second IRES is that of REV-A [22], while in pEMCV-CBT4 it is that of EMCV [66]. In both vectors the second IRES drives the expression of neomycin phosphotransferase (Neo). The pREV-HW1 lacks the packaging sequence and the IRES of MLV and thus it cannot generate recombinant virus [22]. The pREV-HW1 missing the MLV Psi/IRES sequences was used as an internal negative control.

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