Figure 7From: HIV-1 Tat interacts with LIS1 proteinLIS1 interacts with Tat in yeast two-hybrid assay. EGY48 yeast cells were transformed, as described in Experimental procedures, with pSH18–34 reporter and combinations of pLexA and pJG4–5 empty vectors (panel A); pLexA and pJG LIS1 (panel B); pLexA Tat and pJG 4–5 (panel C); pLexA Tat 86 and pJG LIS1 (panel D). Six independent colonies from each transformation were cultured on plates containing Galactose/Raffinose to induce Tat and LIS1 synthesis and X-Gal substrate to detect β-galactosidase.Back to article page