Figure 6

Co-immunoprecipitation of HIV-1 Tat with LIS1 from HeLa cells. HeLa whole cell extracts, with and without Flag-Tat, were prepared from uninfected and Adeno-Tat infected cells as described in the Experimental procedures section. A, LIS1 was immunoprecipitated with monoclonal anti-LIS1 antibodies, resolved by 10% Tris-Tricine gel and immunoblotted with anti-Flag antibodies to detect Flag-Tat. B, Flag-Tat was immunoprecipitated with polyclonal anti-Flag antibodies, resolved by 12% Tris-Tricine gel and probed with monoclonal anti-LIS1 antibodies to detect LIS1.