Figure 4
LIS1 binds to HIV-1 Tat in vitro . Individual protein components of Tat-associated complex were translated in reticulocyte lysate containing [35S]methionine as described in the Experimental procedures section. A, Input lysates, resolved on 12% SDS-PAGE. Lane 1- CDK7; Lane 2-Cyclin H; Lane 3-MAT1; Lane 4-LIS1; Lane 5-the N-terminal domain of LIS1 (LIS NT); Lane 6- WD7; Lane 7-WD6; Lane 8 – WD5; Lane 9 – WD4; Lane 10- WD3; and Lane 11- WD1. B, programmed reticulocyte lysates from panel A precipitated with GST-Tat 72 immobilized on glutathione-agarose beads, and resolved on 12% SDS-PAGE.