Figure 6

Mapping of amino acid residues that are important for the receptor function of human Hyal2 to the bee venom Hya structure. Amino acids of human Hyal2 were mapped to the bee venom Hya protein structure [PDB:1FCV]. Positions at which alteration of the human Hyal2 sequence to that of the mouse resulted in a decrease in receptor activity are colored red, those that resulted in an increase in receptor function are colored green. The hyaluronic acid tetramer that was present in the crystal structure and the active site of the enzyme (blue) can be seen in the middle structure. Protein structure images were generated by using PyMOL [24].