Figure 3

Alignment of Hyal2 protein sequences from different species and effect on receptor function of conversion of amino acid residues in human Hyal2 to those present in mouse Hyal2. ClustalW [23] was used to generate an alignment of ovine [GenBank:AF411974.1], human [GenBank:U09577.1], C3H mouse [GenBank:AF302843.1], and rat [GenBank:AF535141.1] Hyal2 protein sequences. Ovine Hyal2 has a three amino acid insertion following amino acid 56 that is not present in the other Hyal2 proteins. Amino acid identity (*), strong similarity (:), weaker similarity (.), and dissimilarity (no mark) are indicated below the sequences. Downward arrows indicate positions of junctions between chimeric human/mouse Hyal2 chimeras, and dots above the sequences indicate 10 base-pair intervals. Residues that differ between human and mouse Hyal2 in the region between amino acids 158 and 354 are indicated in red, and the receptor activities (% of human Hyal2 activity) of human Hyal2 proteins containing the single or double replacements with the respective mouse amino acids are shown above the amino acid residues. Where two amino acids were altered, a bracket depicts the residues that were altered.