Figure 1

Bispecific lentiviral vector (XHR) encoding anti-CXCR4 and CCR5 shRNAs: A) Control transfer vector pHIV-7-GFP encoding a CMV promoter driven EGFP reporter gene. B) To derive the bispecific vector pHIV-XHR-GFP, a U6 promoter driven short hairpin CXCR4 shRNA cassette was cloned into the BamHI site upstream of the CMV-EGFP cassette. The H1-CCR5 shRNA cassette was inserted into an MluI site downstream to the U6-CXCR4 shRNA cassette.