Figure 1
From: Efficient trapping of HIV-1 envelope protein by hetero-oligomerization with an N-helix chimera
A. Comparison of consensus amino acid sequences of N-helix regions from various HIV-1 clades, and the consensus sequence that was used to make pNH-YFPgpi. The letters a and d under the consensus sequence indicate the position of corresponding amino acids on a helical wheel. B. Schematic diagram of the coding sequence regions in expression plasmids pYFPgpi and pNH-YFPgpi. SP, signal peptide; YFP, yellow fluorescent protein; NH, N-helix; GPI, gpi attachment signal. C. Western blot with anti-GFP antiserum of HeLa cells transfected with pNH-YFPgpi (lane 1), pYFPgpi (lane 2), or untransfected HeLa cells (lane 3). *, aberrant YFPgpi product. One of three independent experiments with similar results is shown.