HIV-1 Tat protein enhances Microtubule polymerization

Retrovirology

Table 1 Enhancement of tubulin polymerization by different Tat and derived peptides

Compounds	Concentration	ΔOD_{350 nm}	ΔOD ratio
none		0.31	1
Tat HxB2	8 μM	1.01	3.31
Tat OYI	8 μM	0.80	2.63
Tat ELI	8 μM	1.61	5.27
Paclitaxel	15 μM	0.55	1.79
Pep38–72 OYI	8 μM	0.78	2.56
Pep38–72 ELI	8 μM	1.59	5.20
Pep73–101 OYI	8 μM	0.31	1.01
Pep73–99 ELI	8 μM	0.30	1

15 μM tubulin is incubated at 37°C in buffer, the increase of ΔOD_{350 nm} is followed and the value of the plateau is indicated in the column ΔOD_{350 nm}. Lane 1 is the control with tubulin alone (none), lanes 2–4 are Tat variants, lane 5 is the paclitaxel control, lanes 6–9 are the Tat peptides. The ΔOD_{350 nm} ratio is the value that the ΔOD_{350 nm} plateau reaches in presence of the indicated product divided by the value of the ΔOD_{350 nm} plateau reached with tubulin alone.

ISSN: 1742-4690