Figure 1

Analysis of N-linked oligosaccharide modification of ASCT1 and ASCT2 with or without PS treatment. (A) NIH 3T3/ASCT2 cells that express HA-tagged human ASCT2 were treated with 400 μM PS for 24 h. Cell lysates were treated with or without PNGase F as described in Materials in Methods, and lysates were analyzed by Western immunoblotting with anti HA-tag monoclonal antibody. (B) 293T cells were transiently transfected with a myc-tagged murine ASCT1 expression plasmid. 400 μM PS was added 24 h post-transfection. Cell lysates were made 48 h post-transfection, were treated with or without PNGase F as described in Materials in Methods, and were analyzed by Western immunoblotting with anti Myc-tag monoclonal antibody.