Figure 3
From: HIV-1 Rev oligomerization is not obligatory in the presence of an extra basic domain
Functional analysis of wild type Rev and M4 mutants by western blot analysis of COS-7 cells co-transfected with the Rev dependent reporter plasmids pDM138-RRE (A, C), pDM138-6xIIB (B, C) or the Rex dependent reporter plasmid pDM138-RxRE (C) together with the plasmids indicated above the lanes. The CAT protein was detected by polyclonal anti-CAT antibodies (Sigma) combined with HRP-labeled anti-rabbit Ig (Amersham) and developed using ECL. The lane numbers are indicated below.