Figure 2From: Dual effect of the SR proteins ASF/SF2, SC35 and 9G8 on HIV-1 RNA splicing and virion productionRegulation of HIV-1 alternative splicing by SR proteins. Analysis of 2 kb (A) and 4 kb (B) mRNAs was performed by RT-PCR using 10 μg of total cellular RNA extracted from 293T cells transfected by HIV-1 pNL4.3 only (lane 1) or together with one SR plasmid (lanes 2–4). Viral mRNAs were identified according to the nomenclature of Purcell and Martin [2].Back to article page