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Figure 1 | Retrovirology

Figure 1

From: Elevated expression of CD30 in adult T-cell leukemia cell lines: possible role in constitutive NF-κB activation

Figure 1

Strategy for cloning NF-κB activating molecules. A) A retroviral cDNA library from an ATL cell line is transduced to a reporter cell line expressing EGFP and bsr in response to NF-κB activation. Blasticidin-resistant and EGFP expressing cell clones are expanded and cDNA clones are obtained by PCR using the retrovirus vector specific primers. B) Visualization of NF-κB activation in reporter cells. Reporter cells were stimulated with TNF-α for 48 hours and tested for the expression of EGFP by FACS analysis.

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