Figure 2

Inactivation of cell-associated HIV-1 in breast milk with SDS. A. Supt-T1 cells infected with HIV-1 IIIB were mixed into breast milk from a healthy donor and treated with 1% or 0.1% SDS for 10 min at 37°C prior to plating on P4-R5 MAGI indicator cells (see methods section for details). Two days later, β-gal expression was measured in relative luminescent units per second (RLU/s) in triplicate samples. Levels of β-gal expression by P4-R5 cells correlates with infectivity of cell-associated HIV-1 (i.e., infected Sup-T1 cells). Results are representative of four experiments. B. Representative results of the time-course of inactivation of cell-associated HIV-1. Sup-T1 cells in media infected with HIV-1 IIIB were treated for up to 15 min with 0.01% SDS and assayed for infectivity using P4-R5 indicator cells. Samples were assayed in triplicate.