Figure 1

Irreversible inactivation of cell-free HIV-1 in breast milk treated with SDS. A. Breast milk from a healthy donor was artificially infected with cell-free HIV-1 IIIB and treated with 0.1% SDS for up to15 min at 37°C prior to plating on P4-R5 MAGI indicator cells (see methods section for details). Two days later, β-gal expression was measured in relative luminescent units per second (RLU/s) in triplicate samples. Results shown are representative of three experiments. B. Infectivity of cell-free HIV-1 in breast milk treated with SDS (0.05% and 0.1%) was assessed before and after removal of SDS with SDS-300 Detergent-Out™ (see methods section for details). Results are representative of two experiments, each with triplicate samples.