Figure 1
Specific binding of gp120:V3 loop peptides to THP-1 and Jurkat cells. (a) Binding of gp120:V3(BaL)-biotin 106 Jurkat cells or THP-1 cells per reaction were incubated with various concentrations of N-terminal biotinylated peptide in the presence and absence of 10 mM unlabelled peptide. Specific binding is expressed as the absorbance in the absence of unlabelled peptide minus the absorbance in the presence of competitor (b) Binding of gp120:V3(IIIb)-biotin under the same conditions as in (a). All reactions were performed in 100 μl of binding medium at 4°C (see Materials and Methods). Values are mean ± SEM from triplicate determinations. * p < 0.05 Student's t-test for specific binding.