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Figure 2 | Retrovirology

Figure 2

From: Preferences for the selection of unique tRNA primers revealed from analysis of HIV-1 replication in peripheral blood mononuclear cells

Figure 2

Replication of HIV-1 with PBS sequence altered to be complementary to the 3' 18 nucleotides of tRNAIle, tRNAMet and tRNAPro in human peripheral blood mononuclear cells (PBMC). Infections were initiated with transfection supernatant containing approximately 200 pg of p24 antigen in a volume of 10 mLs of media, giving a final p24 level of 20 pg/mL on day zero. At 14 day intervals, 5 × 106 fresh PHA stimulated PBMC were added to each culture. Supernatants were assayed for p24 viral antigen using an ELISA. Two additional separate infections produced similar replication patterns for each virus. Squares are wild type NL4-3; diamonds are NL4-3-Met; open circles are NL4-3-Ile and closed circles are NL4-3-Pro.

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