Figure 4

Temporal SOM analysis of HIV-1 Tat-induced cellular genes in synchronized Tat cells. 3 separate filters were applied to remove genes that did not display at least a 1.5, 2, or 3-fold change at each time point analyzed in the 16 eTat chips (see Methods); each filter produced a discrete dataset that was applied to SOM analysis. The third and most restrictive dataset is shown here. Genes that were significantly up (red) and down-regulated (blue) are shown. The U-matrix identifies which genes are similar to each other in terms of expression profile (blue) separated by a "boundary" (red). This SOM graph contains 17 rows and 6 columns of neurons, represented as coordinates. The arrows adjacent to the G₁/S SOM indicate those genes significantly up-regulated during this transition and S phase, and those that show decreased expression in the G₁ phase.