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Table 4 Neutralization of SHIV-2873Nip by plasma samples of SHIVIG recipients

From: Passive immunization of macaques with polyclonal anti-SHIV IgG against a heterologous tier 2 SHIV: outcome depends on IgG dose

Group (mg/kg of SHIVIG)

Monkey

Peak vRNA load (copies/ml)

Day of 1stchallenge

Highest % of neutralization

SHIVIG plasma IC50(μg/ml)

Group 1

RVv-12

4,500

90.1

12.9

(400)

RQv-12

342,850

85.7

10.9

Group 2

RZu-11

201,700

74.7 (91.8)

6.0

(675)

RRs-12

229,600

88.8 (95.6)

4.8

Group 3

RWt-12

61,750

58.5

6.2

(25)

RNz-11

1,032,600

66.7

3.3

  1. The highest percent of neutralization was seen at a plasma dilution of 1:6. Values in parentheses represent the highest % of neutralization reached for RZu-11 and RRs-11 at plasma dilutions 1:54 and 1:18, respectively. SHIVIG plasma IC50 concentrations were determined using the concentration of SHIVIG in RM plasma on the day of challenge and the dilution of the same plasma sample showing 50% of neutralization in PBMC assay. Neutralization assays were performed at least in triplicate. Measurements of viral loads were performed in duplicate.