Passive immunization of macaques with polyclonal anti-SHIV IgG against a heterologous tier 2 SHIV: outcome depends on IgG dose

Table 2 Percent neutralization of different virus strains by SHIVIG in TZM-bl cell-based assay

Virus	Clade	Tier	SHIVIG	^bPositive control
KNH1088.ec5	A	2	0	83
SF162 PV	B	1	94	100
BaL.ec1	B	1	92	99
GS015.ec12	C	1	74	95
GS 014 IMC	C	2	0	82
E0836M4.ec3	D	2	0	80
CM235.ec5	AE	2	66	100
GS 020 IMC	AE	2	0	38
55815.ec3	AG	2	0	75
SHIV-2873Nip	C	2	^a58	^c56

SHIVIG was tested at 0.5 mg/ml, except where noted. ^aNeutralization obtained with 1 mg/ml of SHIVIG. ^bAn HIV-positive serum pool was used as positive control, except as indicated. ^cBnmAb VRC01 (50 μg/ml) was used as positive control. IMC, infectious molecular clone. All neutralization assays were performed at least in duplicate.

ISSN: 1742-4690