Skip to main content

Table 1 The synthetic oligonucleotides uses in this study

From: The dUTPase-related gene of bovine immunodeficiency virus is critical for viral replication, despite the lack of dUTPase activity of the encoded protein

Designations (lengths)

Sequences

Usage

N5A (36nt)

CCATGAGCCATATGGCCCCGTGTAGCCCTCCTGAGG

BIV dUTPase cloning

N5B (41nt)

CCATGCGAATTCAACAGGTCATGAAGACCTCATCTTTTTGG

BIV dUTPase cloning

N6A (33nt)

CCATGAGCCATATGCTAGCATACCAAGGCACAC

EIAV dUTPase cloning

N6B (32nt)

CCTAGCGAATTCCAGAATACTCCTGTACTTCC

EIAV dUTPase cloning

N110 (27nt)

ATCTCTCATGATCTTCAGTGGCTGAGG

BIV sequencing

N111 (26nt)

ATGCGGACATGAACAGTACCTAAGCC

PCR-based dUTPase assay

N120 (52nt)

GAGGTATTACCATGGTAGAGTCAGAGCTACAGCTACAGCTACTTAGCATAGG

BIV dUTPase point mutagenesis

N122 (26nt)

GAAGGCCATATGCATGGCTCTCTTGG

BIV dUTPase deletion mutagenesis

N123 (38nt)

GAATGTTACCATGGTAATGGCCTCAGGAGGGCTACACG

BIV dUTPase deletion mutagenesis

N124 (43nt)

GAATAGTCGACAGGAAATGCGTAGATTGAGGGTGCCTCTAAGG

BIV dUTPase point mutagenesis

N126 (28nt)

CACAATCCCAGGGAGTTGTAGAAAGAGC

BIV sequencing

N142 (20nt)

CCTTCAGCTTCCGTTCTTTC

BIV (qPCR) 93bp upstream to U3

N143 (19nt)

CGGAAGATGTCCCAAAGGA

BIV (qPCR) 33bp upstream to U3

N144 (20nt)

CTCTAATGGCCACTCAGCAA

MLV (qPCR)

N145 (20nt)

CCTCCCTGAGATCATCCTGT

MLV (qPCR)

N156 (23nt)

GACTCTTCTGTTGTATCGGGAAA

EIAV (qPCR)

N157 (18nt)

GGAAGCAAGGGGCTCAAG

EIAV (qPCR)

N162 (21nt)

TGCTGGTTTACCGGTTTATTG

Plasmid Backbone (qPCR)

N163 (20nt)

CAGGCATTTGAGAAGCACAC

Plasmid Backbone (qPCR)

N201 (24nt)

ATTCTAGAGGCCATTACGGCCTCG

Canine repetitive genomic sequence (PCR)

N207 (19nt)

GCCCGTCACCCATTCACCC

Canine repetitive genomic sequence (PCR)

Linker1-BIV (30nt)

TCGAGCACGTGTCTAGAGAATAATTGGGCC

Creating pET-22b(+)-PmlI plasmid

Linker2-BIV (22nt)

CAATTATTCTCTAGACACGTGC

Creating pET-22b(+)-PmlI plasmid