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Figure 5 | Retrovirology

Figure 5

From: The dUTPase-related gene of bovine immunodeficiency virus is critical for viral replication, despite the lack of dUTPase activity of the encoded protein

Figure 5

The amounts of viral particles produced by transfection. Wild-type and mutant BIV were produced by transfecting Cf2Th cells with the viral plasmids. The cells were treated with the following plasmids: pBIV127 (for WT virus), pBIV127 D48E/N57S or pBIV127 Δ36 (for the two BIV mutants), as described in Methods. In short, Cf2Th cells (70-80% confluent) were used for transfection by 4 ug DNA/well in 6 well plates with the TurboFect Transfection Reagent, following the recommended protocols. Cells were harvested 48 hours later and the number of viral particles was evaluated, as described in Methods. Representative data from three independent experiments are shown. The BIV virions are marked according to their dUTPase mutation.

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Retrovirology

ISSN: 1742-4690

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