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Figure 3 | Retrovirology

Figure 3

From: The dUTPase-related gene of bovine immunodeficiency virus is critical for viral replication, despite the lack of dUTPase activity of the encoded protein

Figure 3

The PPiLight pyrophosphate detection assay for the dUTPase enzymatic activities. This assay was performed to assess the dUTPase activity in purified recombinant proteins. For details, see Methods and the text. 25 ng of each recombinant enzyme were incubated at 37°C for 30 min with dUTP-containing reaction buffer that was then terminated by heating for 3 min at 95°C. RLU- relative luminescence units.

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Retrovirology

ISSN: 1742-4690

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