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Figure 2 | Retrovirology

Figure 2

From: The dUTPase-related gene of bovine immunodeficiency virus is critical for viral replication, despite the lack of dUTPase activity of the encoded protein

Figure 2

Western analysis of the purified recombinant BIV dUTPase proteins. The proteins were prepared and purified as described in Methods and in the text. The proteins were analyzed by 10% SDS/PAGE and the gels were then underwent western blot analysis. The blot was treated with 1:500 dilution of rabbit antiserum prepared against the dUTPase-derived peptide (see Methods). After blocking the serum-treated blot, it was treated with a 1:10,000 dilution of a secondary donkey HRP-conjugated anti-rabbit IgG antibody that was purchased from abcam.

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