Figure 7
From: The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3to HIV-1 RNA
Model summarizing the involvement of RHA in multiple steps of HIV-1 RNA metabolism and the proposed role of HAP95 in the annealing of tRNALys3to viral RNA. RHA mainly associates with the structured 5’-UTR of HIV-1 RNA during virus production. RHA stimulates the accumulation of viral RNA in the nucleus and then by remodeling the highly structured 5’-UTR to facilitate both the viral translation and the annealing of tRNALys3 to viral RNA in the cytoplasm, and finally assembles into progeny virions. HAP95 is likely to be employed to transiently block the activity of RHA to protect the annealed tRNALys3 on viral RNA in the cell from being removed by RHA’s activity prior to the budding of virus particles.