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Figure 5 | Retrovirology

Figure 5

From: The role of A-kinase anchoring protein 95-like protein in annealing of tRNALys3to HIV-1 RNA

Figure 5

Effect of knockdown of RHA and HAP95 together upon the annealing of tRNALys3to viral RNA. 293 T cells were first treated with siRNACon, siRNARHA, or siRNAHAP, or with both siRNARHA and siRNAHAP, and 16 hours later, were transfected with DNA coding for HIV-1 BH10. (A) Western blots of cell or viral lysates were probed with antibodies against RHA, HAP95, β-actin, CAp24, or RTp66/p51. (B) +6 nt extension assay was performed as described in Figure 4 legend and the results were presented graphically in lower panel. Shown are the mean values ± SD of 3 experiments. (C) Viral infectivity assay was performed as described in Figure 2 legend. *, P <0.05 compared to values obtained with virions produced from the cells treated with siRNACon, siRNARHA or siRNAHAP alone (lane 1, 2, or 3 respectively).

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